Immunocytochemical localization of galanin in the rat male and female genital tracts and motor effects in vitro
Author
Summary, in English
Galanin, a recently discovered neuropeptide, was studied in the rat male and female reproductive tracts by immunocytochemistry and in vitro pharmacology. Nerve fibers containing galanin immunoreactivity were most abundant in the female paracervical tissue, where they surrounded non-immunoreactive ganglion cells. Galanin nerves were also found in the uterus and Fallopian tubes, as well as in the vas deferens. When tested in vitro galanin contracted the smooth muscle of both the uterine horn and cervix. Galanin also slightly potentiated the response to electrical field stimulation in preparations from the uterine cervix and vas deferens, but it had no effect on the seminal vesicle. Galanin-(1-10), an N-terminal residue of galanin, also contracted the uterine horn, though higher concentrations were required. The neurally induced contractions were not influenced by galanin-(1-10) in any of the smooth muscle preparations tested. The muscle receptors mediating the direct contractile effects in the uterine horn seem to require the N-terminus of galanin, while the neuromodulatory effects on the electrically induced contractile activity seem to need the C-terminal part or the whole galanin molecule. Galanin may thus function as a neuromediator in the rat male and female genital organs.
Department/s
- Urogynaecology and Reproductive Pharmacology
- Neurogastroenterology
- Drug Target Discovery
Publishing year
1988
Language
English
Pages
335-343
Publication/Series
Regulatory Peptides
Volume
20
Issue
4
Document type
Journal article
Publisher
Elsevier
Topic
- Cell and Molecular Biology
Keywords
- Galanin
- Neuropeptide
- Male and female genital tracts
- Uterus
- Vas deferens
- Seminal vesicle
- Smooth muscle
- Immunocytochemistry
- In vitro pharmacology
Status
Published
Research group
- Urogynaecology and Reproductive Pharmacology
- Neurogastroenterology
- Drug Target Discovery
ISBN/ISSN/Other
- ISSN: 1873-1686