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The novel IgD binding protein from Moraxella catarrhalis induces human B lymphocyte activation and Ig secretion in the presence of Th2 cytokines.

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Author

Summary, in English

Moraxella IgD binding protein (MID) is a novel bacterial outer membrane protein with IgD-binding properties. MID was purified from the respiratory pathogen Moraxella catarrhalis and is here shown to have B cell stimulatory properties. Purified MID in the range of 0.01-0.1 microg/ml was optimal to induce a proliferative response in human PBL. MID coupled to Sepharose and formalin-fixed M. catarrhalis preparations induced similar proliferative responses in PBL cultures. MID or MID-Sepharose stimulated purified human peripheral B cells as measured by proliferation. In contrast, MID or MID-Sepharose did not activate T cells. Preincubation of purified B cells with anti-IgD Abs inhibited MID-Sepharose-induced B cell proliferation. The addition of IL-4 specifically induced IL-6 production in MID-Sepharose-activated B cells. IgM secretion was detected in B cell cultures stimulated with MID or MID-Sepharose and IL-2 for 10 days. Secretion of IgG and IgA was efficiently induced in cultures from purified B cells stimulated with the combination of MID or MID-Sepharose and IL-4, IL-10, and soluble CD40 ligand, suggesting that Th2-derived cytokines were required for optimal plasma cell generation. Taken together, MID has properties that make it an important tool to study IgD-targeted activation of B cells.

Publishing year

2002

Language

English

Pages

5582-5588

Publication/Series

Journal of Immunology

Volume

168

Issue

11

Document type

Journal article

Publisher

American Association of Immunologists

Topic

  • Immunology in the medical area

Keywords

  • Immunoglobulin D : physiology
  • Immunoglobulin A : biosynthesis
  • Human
  • Drug
  • Dose-Response Relationship
  • Cytokines
  • Carrier Proteins : pharmacology
  • B-Lymphocytes : immunology
  • B-Lymphocytes : drug effects
  • Interleukin-6 : biosynthesis
  • Lymphocyte Transformation : drug effects
  • Support
  • Non-U.S. Gov't
  • Th2 Cells : immunology
  • Immunoglobulin G : biosynthesis
  • Immunoglobulin M : biosynthesis
  • Interleukin-2 : pharmacology

Status

Published

Research group

  • Clinical Microbiology, Malmö

ISBN/ISSN/Other

  • ISSN: 1550-6606