Signs of Degeneration in 12-22-Year Old Grafts of Mesencephalic Dopamine Neurons in Patients with Parkinson's Disease
Author
Summary, in English
We demonstrate that grafted human fetal mesencephalic neurons can survive and extend axons for 22 years in the brain of a patient with Parkinson's disease (PD). In this patient, the overall survival and fiber outgrowth of the grafts were, however, relatively poor, which is consistent with the lack of significant clinical graft-induced benefit. We have compared the morphology of neurons in the 22-year old grafts with those in two younger grafts (16- and 12-year old), which were sequentially implanted in another PD patient. In the case with the 22-year-old transplant, a high proportion (up to 38%) of the grafted dopaminergic (pigment-granule containing) neurons do not express tyrosine hydroxylase and dopamine transporter and their perikarya appear atrophic. The proportion of pigmented neurons not expressing these markers is lower in the 12-16 year old grafts. Furthermore, in the 22-year-old graft, 49% of the pigmented neurons display alpha-synuclein immunoreactivity in the cell body and 1.2% of them contain Lewy bodies. In conclusion, our results show that grafted dopaminergic neurons can survive for more than two decades. However, over time an increasing proportion of grafted neurons exhibit signs of degeneration.
Department/s
Publishing year
2011
Language
English
Pages
83-92
Publication/Series
Journal of Parkinson's Disease
Volume
1
Issue
1
Document type
Journal article
Publisher
IOS Press
Topic
- Neurology
Keywords
- Neural
- transplantation
- graft survival
- dopamine
- phenotype
- tyrosine
- hydroxylase
- alpha-synuclein
- Lewy bodies
Status
Published
Research group
- Neural Plasticity and Repair
ISBN/ISSN/Other
- ISSN: 1877-718X