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Standardisation and use of the alcohol biomarker carbohydrate-deficient transferrin (CDT)

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Author

  • Anders Helander
  • Jos Wielders
  • Raymond Anton
  • Torsten Arndt
  • Vincenza Bianchi
  • Jean Deenmamode
  • Jan-Olof Jeppsson
  • John B. Whitfield
  • Cas Weykamp
  • François Schellenberg

Summary, in English

Carbohydrate-deficient transferrin (CDT) is a glycoform profile of serum transferrin that increases in response to sustained high alcohol intake and over the last decades has become an important alcohol biomarker with clinical and forensic applications. However, the wide range of CDT measurement procedures has resulted in lack of uniform results and reference limits, and hampered comparison of results. In 2005, the IFCC therefore founded a special working group (WG) aiming for standardisation of CDT measurement. This review summarises the history of CDT and the actions taken by the WG-CDT. Initial steps included the definition of the measurand (serum disialotransferrin to total transferrin fraction expressed in %), and the determination of a well-defined anion-exchange HPLC procedure as the candidate reference measurement procedure (cRMP). Subsequent achievements were the establishment of a network of reference laboratories to perform the cRMP, setting a reference interval, and development of a reference material based on human serum for which the laboratory network assign values. Using a set of reference materials for calibration allowed for achieving equivalence of results of all present CDT measurement procedures. The final steps of the WG-CDT have been a full validation of the cRMP to make it an IFCC approved RMP, and providing guidance for international standardisation of all CDT measurement procedures.

Publishing year

2016-08-01

Language

English

Pages

19-24

Publication/Series

Clinica Chimica Acta

Volume

459

Document type

Journal article

Publisher

Elsevier

Topic

  • Other Clinical Medicine

Keywords

  • Alcohol biomarker
  • Carbohydrate-deficient transferrin
  • CDT
  • HPLC
  • IFCC
  • Serum transferrin
  • Standardisation

Status

Published

ISBN/ISSN/Other

  • ISSN: 0009-8981