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Acoustic actuated fluorescence activated sorting of microparticles.

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Author

Summary, in English

In this paper, we present a fluorescence activated sorter realized in a continuous flow microfluidic chip. Sorting is achieved by deflecting a focused particle stream with short acoustic bursts (2.5 ms), in a fluorescence activated configuration. The system utilizes two-dimensional acoustic pre-focusing, using a single actuation frequency, to position all particles in the same fluid velocity regime at flow rates up to 1.7 mL min(-1). Particles were sorted based on their fluorescence intensities at throughputs up to 150 particles s(-1). The highest purity reached was 80% when sorting at an average rate of 50 particles s(-1). The average recovery of a sort was 93.2 ± 2.6%. The presented system enables fluorescence activated cell sorting in a continuous flow microfluidic format that allows aseptic integration of downstream microfluidic functionalities, opening for medical and clinical applications.

Publishing year

2014

Language

English

Pages

1943-1950

Publication/Series

Lab on a Chip

Volume

14

Issue

11

Document type

Journal article

Publisher

Royal Society of Chemistry

Topic

  • Medical Laboratory and Measurements Technologies

Status

Published

ISBN/ISSN/Other

  • ISSN: 1473-0189