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Cell-specific processing of chromogranin A in endocrine cells of the rat stomach

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Author

  • Per Norlén
  • William J. Curry
  • Maria Björkqvist
  • Aron Maule
  • Rodat T. Cunningham
  • Robert B. Hogg
  • Pat Harriott
  • Colin F. Johnston
  • John C. Hutton
  • Rolf Håkanson

Summary, in English

The rat stomach is rich in endocrine cells. The acid-producing (oxyntic) mucosa contains ECL cells, A-like cells, and somatostatin (D) cells, and the antrum harbours gastrin (G) cells, enterochromaffin (EC) cells and D cells. Although chromogranin A (CgA) occurs in all these cells, its processing appears to differ from one cell type to another. Eleven antisera generated to different regions of rat CgA, two antisera generated to a human (h) CgA sequences, and one to a bovine Ib) CgA sequence, respectively, were employed together with antisera directed towards cell-specific markers such as gastrin (G cells), serotonin (EC cells), histidine decarboxylsae (ECL cells) and somatostatin (D cells) to characterize the expression of CgA and CgA-derived peptides in the various endocrine cell populations of the rat stomach. In the oxyntic mucosa, antisera raised against CgA(291-319) and CGA(316-321) immunostained D cells exclusively, whereas antisera raised against bCgA(82-91) and CgA(121-128) immunostained A-like cells and D cells. Antisera raised against CgA(318-349) and CgA(437-448) immunostained ECL cells and A-like cells, but not D cells. In the antrum, antisera against CgA(291-319) immunostained D cells, and antisera against CgA(351-356) immunostained G cells. Our observations suggest that each individual endocrine cell type in the rat stomach generates a unique mixture of CgA-derived peptides, probably reflecting cell-specific differences in the post-translational processing of CgA and its peptide products. A panel of antisera that recognize specific domains of CgA may help to identify individual endocrine cell populations.

Publishing year

2001

Language

English

Pages

41535-41535

Publication/Series

Journal of Histochemistry and Cytochemistry

Volume

49

Issue

1

Document type

Journal article

Publisher

Histochemical Society

Topic

  • Pharmacology and Toxicology

Keywords

  • chromogranin A
  • ECL cell
  • D cell
  • A-like cell
  • EC cell
  • G cell
  • processing

Status

Published

Research group

  • Translational Neuroendocrinology
  • Drug Target Discovery

ISBN/ISSN/Other

  • ISSN: 0022-1554