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Assignment of allotypes G1m(a+) and G1m(a-) at the genomic level by polymerase chain reaction analysis

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Author

Summary, in English

A method of assignment of the human immunoglobulin allotypes G1m(a+) and G1m(a-) without the use of serological reagents is described. It is based upon oligonucleotide-directed enzymatic amplification of genomic segments encoding CH3 of gamma chains, followed by dot-blot hybridization of radioactively labelled oligonucleotides to the amplified DNA. The method was used to classify the immunoglobulin allotypes of 11 persons, six G1m(a+) and five G1m(a-), and the resultant classification agreed completely with that of classical serological typing.

Publishing year

1990

Language

English

Pages

205-212

Publication/Series

Experimental and Clinical Immunogenetics

Volume

7

Issue

4

Document type

Journal article

Publisher

Karger

Topic

  • Pharmacology and Toxicology
  • Medicinal Chemistry

Status

Published

ISBN/ISSN/Other

  • ISSN: 0254-9670