Experimental Models to Study Drug Distributions in Tissue Using MALDI Mass Spectrometry
Author
Summary, in English
Requirements for patient safety and improved efficacy are steadily increasing in modern healthcare and are key drivers in modern drug development. New drug characterization assays are central in providing evidence of the specificity and selectivity of drugs. Meeting this need, matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) is used to study drug localization within micro-environmental tissue compartments. Thin sections of human lung tumor and rat xenograft tissues were exposed to pharmaceutical drugs by either spotting or submerging. These drugs, the epidermal growth factor receptor antagonists, erlotinib (Tarceva™) and gefitinib (Iressa™), and the acetylcholine receptor antagonist, tiotropium were characterized by microenvironment localization. Intact tissue blocks were also immersed in drug solution followed by sectioning. MALDI-MSI was then performed using a Thermo MALDI LTQ Orbitrap XL instrument to localize drug distribution patterns. We propose three MALDI-MSI models measuring drug disposition that have been used to map the selected compounds within tissue compartments of tumors isolated from lung cancer patients.
Department/s
- Department of Biomedical Engineering
- Department of Clinical Sciences, Lund
- Tumor microenvironment
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publishing year
2013
Language
English
Pages
5626-5633
Publication/Series
Journal of Proteome Research
Volume
12
Issue
12
Document type
Journal article
Publisher
The American Chemical Society (ACS)
Topic
- Medical Engineering
Keywords
- Lung Cancer
- Adenocarcinoma
- MALDI-Mass Spectrometry Imaging
- Erlotinib
- Gefitinib
- Tiotropium
Status
Published
ISBN/ISSN/Other
- ISSN: 1535-3893