Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays
Author
Summary, in English
Antibody microarrays enable extensive protein expression profiling, and provide a valuable complement to DNA microarray-based gene expression profiling. In this study, we used DotScan™ antibody microarrays that contain antibodies against 82 different cell surface antigens, to determine phenotypic protein expression profiles for human B cell sub-populations. We then demonstrated that the B cell protein profile can be used to delineate the relationship between normal B cells and malignant counterparts. Principle component analysis showed that the lymphomas did not cluster with the normal memory B cells or germinal centre B cells, but they did cluster with germinal centre founder cells and naïve B cells.
Department/s
Publishing year
2008
Language
English
Pages
98-106
Publication/Series
Cancer Letters
Volume
265
Document type
Journal article
Publisher
Elsevier
Topic
- Cancer and Oncology
Status
Published
Research group
- Carl Borrebaeck
ISBN/ISSN/Other
- ISSN: 1872-7980