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Complimentary action : C1q increases ganglion cell survival in an in vitro model of retinal degeneration

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Author

Summary, in English

Using a previously described retinal explant culture system as an acute injury model, we here explore the role of C1q, the initiator of the classical complement pathway, in neuronal cell survival and retinal homeostasis. Full-thickness adult rat retinal explants were divided into four groups, receiving the following supplementation: C1q (50 nM), C1-inhibitor (C1-inh; Berinert; 500 mg/l), C1q + C1-inh, and no supplementation (culture controls). Explants were kept for 12 h or 2 days after which they were examined morphologically and with a panel of immunohistochemical markers. C1q supplementation protects ganglion cells from degeneration within the explant in vitro system. This effect is correlated to an attenuated endogenous production of C1q, and a quiesced gliotic response.

Publishing year

2016-09-15

Language

English

Pages

117-129

Publication/Series

Journal of Neuroimmunology

Volume

298

Document type

Journal article

Publisher

Elsevier

Topic

  • Cell and Molecular Biology
  • Ophthalmology

Keywords

  • Adult retina
  • C1q
  • Ganglion cell
  • Neuroinflammation
  • Retinal degeneration
  • Retinal explant culture

Status

Published

Research group

  • Protein Chemistry, Malmö

ISBN/ISSN/Other

  • ISSN: 0165-5728