Coreceptor usage of primary HIV type 1 isolates obtained from different lymph node subsets

Summary, in English

Biological characteristics of virus quantitatively rescued from different cell types present in lymph nodes of HIV-1-infected individuals in various stages of their disease were determined, not including patients with AIDS defining illness. Viruses were obtained by cocultivation with donor monocyte-derived macrophages and T-lymphocytes and their biological phenotype compared to viruses obtained from the peripheral blood mononuclear cells of the same patient. The biological phenotype was determined on established cell lines (U937-2, CEM, and MT-2) and on the U87.CD4 coreceptor indicator cell lines and variable region 3 (V3) of the envelope was subjected to direct sequencing. All isolates obtained from lymph node subsets used CCR5 as coreceptor. Furthermore, these viruses were also sensitive to inhibition by beta-chemokines as analyzed for viruses of one patient. All 12 V3 regions showed a unique sequence indicating compartmentalization within each patient. The biological phenotype of CCR5-dependent (R5) HIV-1 isolates obtained from PBMC resembles the phenotype of viruses isolated from different lymph node cell subsets.

• Author: C Casper; Mattias Mild; Marianne Jansson; A Karlsson; V Holmberg; G Bratt; H van Paaschen; P Biberfeld; A Bjorndal; J Albert; M Popovic; Eva Maria Fenyö
• Department/s: Division of Medical Microbiology
• Publishing year: 2005
• Language: English
• Pages: 1003-1010
• Publication/Series: AIDS Research and Human Retroviruses
• Volume: 21
• Issue: 12
• Links: http://dx.doi.org/10.1089/aid.2005.21.1003
• Document type: Journal article
• Publisher: Mary Ann Liebert, Inc.
• Topic: Microbiology in the medical area
• Status: Published
• ISBN/ISSN/Other: ISSN: 1931-8405