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Evidence for disease-regulated transgene expression in the brain with use of lentiviral vectors.

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Author

Summary, in English

In this study we have developed and validated a novel approach of transgene regulation in the brain. By using lentiviral vectors that incorporate promoters of genes that are up-regulated during different pathological states, we were able to regulate transgene expression in accordance with the disease process. When using a glial fibrillary acidic protein promoter, efficient disease regulation in glial cells was achieved after an excitotoxic lesion or a 6-hydroxydopamine (6-OHDA) lesion. Transgene expression was physiologically regulated and displayed a dose-dependent increase depending on the severity of lesion. Efficient regulation was also achieved in neurons when using a preproenkephlin promoter in 6-OHDA-lesioned rats, allowing combined regulation and targeting. This disease-regulated approach allows control of transgene expression in the brain without the use of inducer molecules and without overexpression of transactivator proteins

Publishing year

2006

Language

English

Pages

58-67

Publication/Series

Journal of Neuroscience Research

Volume

84

Issue

1

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Neurosciences

Keywords

  • disease-regulated transgene expression
  • brain
  • lentiviral vectors

Status

Published

Research group

  • Brain Repair and Imaging in Neural Systems (BRAINS)
  • CNS Gene Therapy

ISBN/ISSN/Other

  • ISSN: 1097-4547