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Production of prebiotic xylooligosaccharides from alkaline extracted wheat straw using the K80R-variant of a thermostable alkali-tolerant xylanase

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Author

Summary, in English

Agricultural by-products are raw materials of importance for increased utilization of renewable biomass. Wheat straw is a raw material of significant production volume and is in this work used for production of xylooligosaccharides (XOS). Extraction of xylan by dilute alkali was followed by hydrolysis using a variant of the alkali-tolerant Bacillus halodurans S7 endoxylanase A mutated at K80R. The xylan yield was on average 56.5 g xylose equivalents per kg dried, ground wheat straw, with 1 arabinose per 12 xylose residues. The K80R variant, which displayed higher specific activity than the wild-type enzyme, was added at a load of 96 U/g extracted xylan. The XOS-yield (xylobiose – xylopentaose) was evaluated at time intervals in the temperature range of 50 to 65 degrees C, at pHs from 7 to 10. The enzyme was optimally active at 60 degrees C up to pH 9. Hydrolysis was completed within 7 h, resulting in 36 % conversion of the xylan to predominantly xylobiose. Xylose content was low (2.4%) despite extended incubation, which is desirable for XOS-production. The XOS-containing hydrolysate was confirmed as a suitable carbon source for the putative probiotic strain Lactobacillus brevis DSM 1269, showing the applicability of the method to obtain prebiotic XOS.

Publishing year

2015

Language

English

Pages

1-10

Publication/Series

Food and Bioproducts Processing

Volume

93

Issue

Online 22 November 2014

Document type

Journal article

Publisher

Elsevier

Topic

  • Industrial Biotechnology

Keywords

  • XOS
  • Bacillus halodurans
  • Glycoside hydrolase family 10
  • GH10
  • Lactobacillus brevis
  • arabinoxylan

Status

Published

ISBN/ISSN/Other

  • ISSN: 1744-3571