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Leukotriene D(4) induces stress-fibre formation in intestinal epithelial cells via activation of RhoA and PKCdelta.

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Author

Summary, in English

The intestinal epithelial barrier, which is regulated by the actin cytoskeleton, exhibits permeability changes during inflammation. Here we show that activation of the CysLT(1) receptor by the inflammatory mediator leukotriene D(4) (LTD(4)) causes a rapid increase in stress-fibre formation in intestinal epithelial cells. This effect was mimicked by cytotoxic necrotising factor-1 (CNF-1)-induced activation of RhoA, overexpression of constitutively active RhoA (L63-RhoA) and phorbol-ester-induced activation of protein kinase C (PKC). In accordance, inhibition of RhoA, by C3 exoenzyme or by dominant-negative RhoA (N19-RhoA), as well as GF109203X-induced inhibition of PKC, suppressed the LTD(4)-induced stress-fibre formation. Introduction of the dominant-negative regulatory domain of PKCdelta, but not the corresponding structures from PKCalpha, betaII or epsilon, blocked the LTD(4)-induced stress-fibre formation. Evaluating the relationship between PKCdelta and RhoA in LTD(4)-induced stress-fibre formation, we found that C3 exoenzyme inhibited the rapid LTD(4)-elicited translocation of PKCdelta to the plasma membrane. Furthermore, CNF-1-induced stress-fibre formation was blocked by GF109203X and by overexpression of the regulatory domain of PKC-delta, whereas PKC-induced stress-fibre production was not affected by N19-RhoA. We conclude that PKC-delta is located downstream of RhoA and that active RhoA and PKCdelta are both necessary for LTD(4)-induced stress-fibre formation.

Publishing year

2002

Language

English

Pages

15-3509

Publication/Series

Journal of Cell Science

Volume

115

Issue

Pt 17

Document type

Journal article

Publisher

The Company of Biologists Ltd

Topic

  • Cancer and Oncology

Status

Published

ISBN/ISSN/Other

  • ISSN: 0021-9533