Hormonemate, a new cytotoxic and apoptosis-inducing compound from the endophytic fungus Hormonema dematioides. I. Identification of the producing strain, and isolation and biological properties of hormonemate
Author
Summary, in English
In a search for new fungal compounds inducing apoptosis of the colon cancer derived cell line COLO-320, hormonemate (1) was purified from fermentations of an endophytic fungus isolated from living needles of a Pinus species. The producing strain was identified as Hormonema dematioides by microscopy and ITS rDNA sequence analysis. The structure of hormonemate was determined by spectroscopic methods. The compound exhibited cytotoxic effects against the human colon tumor cell lines COLO-320, DLD-1 and HT-29 as well as five other human cell lines (HL-60, JURKAT, HEP-G2, MCF-7, HeLa S3). It also induced apoptosis in COLO-320 cells as detected by a caspase-activity assay and morphological changes, and it triggered morphological and physiological differentiation of HL-60 cells into granulocytes, which subsequently died by apoptosis.
Department/s
Publishing year
2003
Language
English
Pages
547-552
Publication/Series
Zeitschrift fuer Naturforschung. Section C: A Journal of Biosciences
Volume
58
Issue
7-8
Document type
Journal article
Publisher
Verlag der Zeitschrift für Naturforschung
Topic
- Organic Chemistry
Keywords
- hormonemate
- endophyte
- Hormonema dematioides
Status
Published
ISBN/ISSN/Other
- ISSN: 0939-5075