Behavioral characterization of a unilateral 6-OHDA-lesion model of Parkinson's disease in mice.
Author
Summary, in English
Parkinson's disease (PD) is one of the most common neurodegenerative disorders. Several toxin-induced animals models simulate the motor deficits occurring in PD. Among them, the unilateral 6-hydroxydopamine (6-OHDA) model is frequently used in rats and has the advantage of presenting side-biased motor impairments. However, the behavioral consequences of a unilateral 6-OHDA-lesion have, so far, not been described in detail in mice. The aim of this study was to characterize mice with unilateral 6-OHDA-lesions placed in the median forebrain bundle using several motor behavioral tests in order to identify the most suitable predictor of nigral cell loss. Mice underwent various drug-induced (amphetamine- and apomorphine-induced rotation) and spontaneous motor tests (cylinder, rotarod, elevated body swing, and stride length test). The amphetamine-induced rotation test, the cylinder and the rotarod test were most sensitive and reliable in detecting loss of tyrosine hydroxylase-immunoreactive cells in the substantia nigra. This study demonstrates that substantial and stable unilateral 6-OHDA-induced lesions can be established in mice, and that these lesions can be functionally assessed using several different side-bias-based behavioral tests. This mouse model offers the opportunity to use transgenic mouse strains and study the interactions between genes of interest and toxins in relation to Parkinson's disease etiology in the future.
Department/s
- Department of Experimental Medical Science
- Wallenberg Neuroscience Centre, Lund
Publishing year
2005
Language
English
Pages
1-10
Publication/Series
Behavioural Brain Research
Volume
162
Issue
1
Full text
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Document type
Journal article
Publisher
Elsevier
Topic
- Neurosciences
Keywords
- Parkinson disease
- Mouse model
- 6-OHDA
- Behavioral tests
- Tyrosine hydroxylase
Status
Published
ISBN/ISSN/Other
- ISSN: 0166-4328