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Levels Of Bacterial Endotoxin In The Samples Of Settled Dust Collected In Animal Houses

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Author

  • Dorota Pomorska
  • Lennart Larsson
  • Czeslawa Skorska
  • Jolanta Sitkowska
  • Jacek Dutkiewicz

Summary, in English

Samples of settled dust were collected in 14 animal houses and examined for the presence of bacterial endotoxin with the Limulus (LAL) test and the gas chromatography - tandem mass spectrometry (GC-MSMS) technique, based oil the detection of 3-hydroxy fatty acids (3-OH-FAs) as chemical markers of the endotoxin lipopolysaccharide. The median concentrations of the endotoxin in dust determined with LAL test in sheep sheds, poultry houses, and horse stables were 15,687.5 mu g/g, 8,081.8 mu g/g, and 79.3 mu g/g, respectively, while those determined with the GC-MSMS technique were 868.0 mu g/g, 580.0 mu g/g, and 496.0 mu g/g, respectively. Statistical comparison of the results yielded with LAL test and GC-MSMS technique revealed a weak correlation between both methods. Fatty acids with 14-16 carbon chains (3-OH-C-14 and 3-OH-C-16) were predominant in the settled dust of the facilities under study. In conclusion, endotoxin in the concentrations detected in this study may present it respiratory hazard to both livestock animals and farm workers. Thus, the prevention measures aiming to lower the exposure to endotoxin in livestock facilities are highly desirable.

Publishing year

2009

Language

English

Pages

37-41

Publication/Series

Bulletin of the Veterinary Institute in Puławy

Volume

53

Issue

1

Document type

Journal article

Publisher

National Veterinary Research Institute

Topic

  • Microbiology in the medical area

Keywords

  • chromatography-mass spectrometry
  • gas
  • settled dust
  • bacterial endotoxin
  • animal houses
  • Limulus test

Status

Published

ISBN/ISSN/Other

  • ISSN: 0042-4870