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Overexpression of heat shock protein 70 in R6/2 Huntington's disease mice has only modest effects on disease progression.

På svenska

Author

  • Oskar Hansson
  • Jesper Nylandsted
  • Roger F Castilho
  • Marcel Leist
  • Marja Jäättelä
  • Patrik Brundin

Summary, in English

Huntington’s disease (HD) is a neurodegenerative disorder caused by expansion of a polyglutamine tract in a protein called huntingtin. The inducible form of heat shock protein 70 (Hsp70) has been shown to reduce polyglutamine-induced toxicity. To investigate if overexpression of Hsp70 can affect disease progression in a mouse model of HD, we crossed R6/2 mice, expressing exon 1 of the HD gene with an expanded CAG repeat, with mice overexpressing Hsp70 (both types of transgenic mice were of the CBAxC57BL/6 strain). The resulting R6/2-Hsp70 transgenics exhibited 5- to 15-fold increases in Hsp70 expression in neocortical, hippocampal and basal ganglia regions. This correlated with a delayed loss of body weight compared to R6/2 mice. However, the number or size of nuclear inclusions, the loss of brain weight, reduction of striatal volume, reduction in size of striatal projection neurons, downregulation of DARPP-32, development of paw clasping phenotype and early death of the mice were not affected by Hsp70 overexpression. Interestingly, the polyglutamine protein affected the potential rescuing agent, because in older R6/2-Hsp70 mice a large proportion of the Hsp70 protein was sequestrated in nuclear inclusions.

Publishing year

2003

Language

English

Pages

47-57

Publication/Series

Brain Research

Volume

970

Issue

1-2

Document type

Journal article

Publisher

Elsevier

Topic

  • Neurosciences

Keywords

  • Heat shock protein 70 (Hsp70)
  • Inclusion
  • Huntington’s disease
  • Chaperone
  • Transgenic
  • Mouse

Status

Published

ISBN/ISSN/Other

  • ISSN: 1872-6240