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Development of an MRM assay panel with application to biobank samples from patients with Myocardial Infarction.

På svenska

Author

Summary, in English

As part of a Swedish national cardiological research initiative, the development of a quantitative MRM assay is reported for the quantification of eleven putative cardiovascular disease markers. Within the study, patient samples from the LUNDHEARTGENE biobank were processed and nanoLC-MS/MS analysis were performed together with stable isotope dilution strategy for absolute quantification of the target proteins. Excellent linear regressions were achieved for 9 of the 11 peptides with LOQ ranged in the attomolar range. We have utilized the assay for the screening of plasma samples from patients with chest pain, and performed a comparative analysis of patients with ST Segment Elevation Myocardial Infarction and chest pain due to other causes. The assay demonstrates high reproducibility and correlate with clinical findings. Strong correlations were found for several of the apolipoproteins and their respective lipid subfractions (LDL, HDL or triglycerides). ApoC1, apoC2 and apoE were elevated in patients with STEMI. BIOLOGICAL SIGNIFICANCE: MRM assay were developed for putative cardiovascular disease markers as target proteins, and applied to biobanking sample material. The comparative analysis of patients with ST Segment Elevation Myocardial Infarction and chest pain due to other causes shows elevated levels of apoC1, apoC2 and apoE in patients with STEMI. These observations raise interesting novel hypotheses about the role of apolipoproteins C1, C2 and E in the pathophysiology of acute myocardial infarction, which merits further studies.

Publishing year

2013

Language

English

Pages

16-25

Publication/Series

Journal of Proteomics

Volume

87

Issue

1

Document type

Journal article

Publisher

Elsevier

Topic

  • Medical Engineering

Keywords

  • Myocardial infarction
  • Multiple reaction monitoring
  • Apolipoproteins

Status

Published

ISBN/ISSN/Other

  • ISSN: 1874-3919