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Increased beta-cell volume in mice fed a high-fat diet A dynamic study over 12 months

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Author

Summary, in English

As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with beta-cell expansion, we have now examined beta-cell volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta-cell volume was estimated as beta-cell area per islet, individual beta-cell size and beta-cell number per islet. Control animals were fed a normal chow (11% fat). We found that beta-cell area per islet and total number of beta-cells per islet were increased already after three months of high-fat feeding and that this increase was sustained throughout the twelve month study period. In contrast, individual beta-cell size showed a dynamic pattern with a reduction after three months followed by increase after six and twelve months. The number of apoptosis (caspase-3) positive beta-cells was reduced after three months, whereas there was no difference in proliferation (Ki-67) positive cells, although these were generally rarely observed. Thus, we conclude that insulin resistance accompanying high-fat feeding in mice is followed by progressive beta-cell expansion as evident by early increased islet beta-cell volume and total number of beta-cells, whereas individual beta-cell size showed a dynamic response. The model is also associated with an early reduced apoptosis, which may contribute to the increased beta-cell volume.

Publishing year

2010

Language

English

Pages

353-356

Publication/Series

Islets

Volume

2

Issue

6

Document type

Journal article

Publisher

Landes Bioscience

Topic

  • Endocrinology and Diabetes

Keywords

  • beta cell mass
  • insulin resistance
  • islet adaptation
  • apoptosis
  • proliferation

Status

Published

ISBN/ISSN/Other

  • ISSN: 1938-2022