The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

New and simple procedure for the determination of muramic acid in chemically complex environments by gas chromatography-ion trap tandem mass spectrometry

På svenska

Author

Summary, in English

A gas chromatographic-ion trap tandem mass spectrometric method was developed for the quantification of muramic acid, a marker of bacterial peptidoglycan, in environmental and clinical specimens. Samples (bacteria, house dust and urine) were heated in methanolic hydrochloric acid overnight and extracted with hexane for removal of hydrophobic compounds. The aqueous phase was evaporated and heated in acetic anhydride and pyridine after which the product, the acetate derivative, was washed with dilute hydrochloric acid and water. The described method is both rapid and simple to apply, and produces a stable derivative. It should become widely used for measuring peptidoglycan in chemically complex environments.

Publishing year

2000

Language

English

Pages

57-65

Publication/Series

Journal of Chromatography. B

Volume

738

Issue

1

Document type

Journal article

Publisher

Elsevier

Topic

  • Microbiology in the medical area

Keywords

  • Muramic acid

Status

Published

ISBN/ISSN/Other

  • ISSN: 1387-2273