Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique

Summary, in English

An immunoelectron microscopic technique using protein A-gold as a specific marker was used for precise intracellular localization of eosinophil granule proteins. Eosinophils from healthy individuals were isolated in metrizamide gradients. Eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were clearly located in the matrix of the large crystalloid-containing granules. In addition, ECP was probably present in the small granules of eosinophils. Major basic protein (MBP) was present in the crystalloid structure of specific granules. This method can be applied in studies of eosinophil degranulation to trace the release of biological effector molecules.

• Author: Arne Egesten; Jan Alumets; C von Mecklenburg; M Palmegren; Inge Olsson
• Department/s: Infection Medicine (BMC); Respiratory Medicine, Allergology, and Palliative Medicine; Tumor microenvironment; Division of Hematology and Transfusion Medicine
• Publishing year: 1986
• Language: English
• Pages: 1399-1403
• Publication/Series: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
• Volume: 34
• Issue: 11
• Links: http://www.ncbi.nlm.nih.gov/pubmed/3772075; http://jhc.sagepub.com/content/34/11/1399.long
• Document type: Journal article
• Publisher: Histochemical Society
• Topic: Medical and Health Sciences
• Status: Published
• ISBN/ISSN/Other: ISSN: 1551-5044