Cystometric findings in mice lacking muscarinic M2 or M3 receptors
Author
Summary, in English
Purpose: The physiological importance of muscarinic M-3 and M-2 receptors for bladder function was investigated in vivo using mice lacking M-3 or M-2 receptors and littermate WT controls. Materials and Methods: Unanesthetized mice of each sex underwent continuous cystometry before and after administration of atropine (1 mg/kg(-1)). Results: Male M-3 knockout (KO) mice had longer voiding intervals, and larger micturition volumes and bladder capacity than M-2 KO or WT males. There was no significant difference in any cystometric parameters between male M-2 KO and WT mice. In females M-3 KO and M-2 KO mice had longer voiding intervals and larger micturition volumes than WT animals. Atropine had marked inhibitory effects on voiding efficacy in WT and M-2 KO mice but it had no effect on any cystometric parameters in M-3 KO mice. Conclusions: The current results confirm that M-3 receptor is the principal muscarinic receptor subtype responsible for bladder contraction and the role of M-2 receptors is of minor importance. Functional impairments found in M-3 KO mice were milder than those elicited by acute blockade of muscarinic receptors by atropine in WT mice, suggesting that noncholinergic mechanisms can compensate for a chronic loss of M-3 receptors.
Department/s
Publishing year
2004
Language
English
Pages
2460-2464
Publication/Series
Journal of Urology
Volume
172
Issue
6, Part 1 of 2
Links
Document type
Journal article
Publisher
Lippincott Williams & Wilkins
Topic
- Urology and Nephrology
Keywords
- receptors
- bladder
- purinergic
- muscarinic
- atropine
- mice
Status
Published
ISBN/ISSN/Other
- ISSN: 1527-3792