Exploring central and peripheral diversity in antibody evolution.
Author
Summary, in English
The antigen-binding site, the paratope, of an antibody can be seen as being composed of a central core and a more peripheral area situated at its rim. Naturally these regions acquire their diversity using different mechanisms and they also have dissimilar roles, as they contribute differently to the binding interaction. Also, antigens of different size utilize these regions differently; while haptens mainly interact with the central core, larger antigens have additional interactions in more peripheral regions. Since haptens do not occupy the entire available paratope we hypothesized that hapten-specific antibodies, as they develop naturally or in the laboratory, have an imprint of the carrier protein they were once selected on. By using combinatorial library and phage display technologies on a hapten-specific antibody we were able to demonstrate that a peripheral carrier imprint indeed exists. We further show that such an imprint can act as a seed in the evolution of binders that recognize the carrier protein even in the absence of the hapten modification. The observed results provide a plausible mechanism for how haptenization of self-antigens can lead to the development of autoimmunity.
Department/s
Publishing year
2007
Language
English
Pages
2729-2736
Publication/Series
Molecular Immunology
Volume
44
Issue
10
Document type
Journal article
Publisher
Pergamon Press Ltd.
Topic
- Immunology in the medical area
Keywords
- Autoimmunity
- Hapten
- Antibody library
- Antibody evolution
- Phage display
- Paratope
Status
Published
ISBN/ISSN/Other
- ISSN: 1872-9142