Improved porous silicon microarray based prostate specific antigen immunoassay by optimized surface density of the capture antibody
Author
Summary, in English
Enriching the surface density of immobilized capture antibodies enhances the detection signal of antibody sandwich microarrays. In this study, we improved the detection sensitivity of our previously developed P-Si (porous silicon) antibody microarray by optimizing concentrations of the capturing antibody. We investigated immunoassays using a P-Si microarray at three different capture antibody (PSA - prostate specific antigen) concentrations, analyzing the influence of the antibody density on the assay detection sensitivity. The LOD (limit of detection) for PSA was 2.5 ng mL(-1), 80 pg mL(1), and 800 fg mL(-1) when arraying the PSA antibody, H117 at the concentration 15 mu g mL(-1), 35 mu g mL(-1), and 154 mu g mL(-1), respectively. We further investigated PSA spiked into human female serum in the range of 800 fg mL(-1) to 500 ng mL(-1). The microarray showed a LOD of 800 fg mL(-1) and a dynamic range of 800 fg mL(-1) to 80 ng mL(-1) in serum spiked samples. (C) 2013 Elsevier B.V. All rights reserved.
Department/s
- Clinical Chemistry, Malmö
- Department of Biomedical Engineering
- EpiHealth: Epidemiology for Health
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publishing year
2013
Language
English
Pages
108-114
Publication/Series
Analytica Chimica Acta
Volume
796
Document type
Journal article
Publisher
Elsevier
Topic
- Analytical Chemistry
Keywords
- Antibody microarray
- Porous silicon
- Sandwich immunoassay
- Prostate
- specific antigen
Status
Published
Research group
- Clinical Chemistry, Malmö
ISBN/ISSN/Other
- ISSN: 1873-4324