Versican V2 Assembles the Extracellular Matrix Surrounding the Nodes of Ranvier in the CNS
Author
Summary, in English
The CNS-restricted versican splice-variant V2 is a large chondroitin sulfate proteoglycan incorporated in the extracellular matrix surrounding myelinated fibers and particularly accumulating at nodes of Ranvier. In vitro, it is a potent inhibitor of axonal growth and therefore considered to participate in the reduction of structural plasticity connected to myelination. To study the role of versican V2 during postnatal development, we designed a novel isoform-specific gene inactivation approach circumventing early embryonic lethality of the complete knock-out and preventing compensation by the remaining versican splice variants. These mice are viable and fertile; however, they display major molecular alterations at the nodes of Ranvier. While the clustering of nodal sodium channels and paranodal structures appear in versican V2-deficient mice unaffected, the formation of the extracellular matrix surrounding the nodes is largely impaired. The conjoint loss of tenascin-R and phosphacan from the perinodal matrix provide strong evidence that versican V2, possibly controlled by a nodal receptor, organizes the extracellular matrix assembly in vivo.
Department/s
Publishing year
2009
Language
English
Pages
7731-7742
Publication/Series
Journal of Neuroscience
Volume
29
Issue
24
Document type
Journal article
Publisher
Society for Neuroscience
Topic
- Neurosciences
Status
Published
Research group
- Vessel Wall Biology
ISBN/ISSN/Other
- ISSN: 1529-2401