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Cortex synthesis during Bacillus subtilis sporulation depends on the transpeptidase activity of SpoVD.

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Author

Summary, in English

The nonessential process of peptidoglycan synthesis during Bacillus subtilis sporulation is one model to study bacterial cell wall biogenesis. SpoVD is a class B high-molecular weight penicillin-binding protein that is specific for sporulation. Strains lacking this protein produce spores without the peptidoglycan cortex layer and are heat-sensitive. The detailed functions of the four different protein domains of the SpoVD protein are unknown and the observed phenotype of strains lacking the entire protein could be an indirect defect. We therefore inactivated the transpeptidase domain by substitution of the active site serine residue. Our results demonstrate that endospore cortex synthesis depends on the transpeptidase activity of SpoVD specifically. This article is protected by copyright. All rights reserved.

Publishing year

2013

Language

English

Pages

65-72

Publication/Series

FEMS Microbiology Letters

Volume

346

Issue

1

Document type

Journal article (letter)

Publisher

Oxford University Press

Topic

  • Biological Sciences

Keywords

  • penicillin-binding protein
  • peptidoglycan
  • endospore.

Status

Published

ISBN/ISSN/Other

  • ISSN: 1574-6968