Signaling to localized degranulation in neutrophils adherent to immune complexes.

Summary, in English

The present study demonstrates that the secretion of azurophilic granules occurring during Fc receptor-mediated attachment and spreading of neutrophils is highly localized to the adhering region of the cell. In contrast, the secretion of specific granules occurs in a nonpolarized way. This implies that unique signals are involved in the regulation of azurophilic degranulation. Assembly of actin filaments, as visualized by staining with rhodamine phalloidin, neither hindered nor facilitated degranulation. Further, the azurophilic secretory response remained localized in the presence of cytochalasin B. Release of azurophilic-granule content was inhibited by genistein and erbstatin, inhibitors of tyrosine kinases, and by GF109203X, a protein kinase C (PKC) inhibitor. We could also demonstrate a relative enrichment of syk tyrosine kinase and the PKC isoforms alpha and beta1 in adherent plasma membranes.

• Author: Claes Nauclér; Sergio Grinstein; Roger Sundler; Hans Tapper
• Department/s: Infection Medicine (BMC); Department of Experimental Medical Science
• Publishing year: 2002
• Language: English
• Pages: 701-710
• Publication/Series: Journal of Leukocyte Biology
• Volume: 71
• Issue: 4
• Links: http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11927658&dopt=Abstract
• Document type: Journal article
• Publisher: John Wiley & Sons Inc.
• Topic: Cell and Molecular Biology
• Keywords: Cell Adhesion; Cell Degranulation; Cytochalasin B : pharmacology; Enzyme Precursors : analysis; Human; Isoenzymes : analysis; Protein Kinase C : analysis; Neutrophils : physiology; Protein Kinase C : physiology; Protein-Tyrosine Kinase : analysis; Protein-Tyrosine Kinase : physiology; Antigen-Antibody Complex : physiology; Actins : metabolism
• Status: Published
• ISBN/ISSN/Other: ISSN: 1938-3673