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Comparison of bacterial diversity along the human intestinal tract by direct cloning and sequencing of 16S rRNA genes

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Author

Summary, in English

Bacterial diversity of the mucosal biopsies from human jejunum, distal ileum, ascending colon and rectum were compared by analysis of PCR-amplified 16S rDNA clone libraries. A total of 347 clones from the mucosal biopsies were partially sequenced and assigned to six phylogenctic phyla of the domain Bacteria: Firmicutes, Bacteroidetes, Proteobacteria, Fusobacteria, Verrucomicrobia, and Actinobacteria. The jejunum sample had least microbial diversity compared to the other samples and a trend towards highest diversity in ascending colon was observed. The clone libraries of distal ileum, ascending colon and rectum were not significantly different from each other (P > 0.0043), but they differed significantly from the jejunum library (P = 0.001). The population of sequences retrieved from jejunal biopsies was dominated by sequences closely related to Streptococcus (67%), while the population of sequences derived from distal ileum, ascending colon and rectum were dominated by sequences affiliated with Bacteroidetes (27-49%), and Clostridium clusters XlVa (20-34%) and IV (7-13%). The results indicate that the microbial community in jejunum is different from those in distal ileum, ascending colon and rectum, and that the major phylogenetic groups are similar from distal ileum to rectum. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Department/s

  • Department of Food Technology, Engineering and Nutrition
  • Surgery

Publishing year

2005

Language

English

Pages

219-231

Publication/Series

FEMS Microbiology Ecology

Volume

54

Issue

2

Document type

Journal article

Publisher

Oxford University Press

Topic

  • Surgery

Keywords

  • Human mucosal biopsy
  • 16S rDNA cloning and sequencing
  • Bacterial diversity

Status

Published

Research group

  • Surgery

ISBN/ISSN/Other

  • ISSN: 1574-6941