The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Extraction of Antioxidants from Spruce (Picea abies) Bark using Eco-friendly Solvents

På svenska

Author

  • Michelle Co
  • Amelie Fagerlund
  • Lars Engman
  • Kerstin Sunnerheim
  • Per J. R. Sjoberg
  • Charlotta Turner

Summary, in English

Introduction - Antioxidants are known to avert oxidation processes and they are found in trees and other plant materials. Tree bark is a major waste product from paper pulp industries; hence it is worthwhile to develop an extraction technique to extract the antioxidants. Objective - To develop a fast and environmentally sustainable extraction technique for the extraction of antioxidants from bark of spruce (Picea abies) and also to identify the extracted antioxidants that are abundant in spruce bark. Methodology - A screening experiment that involved three different techniques was conducted to determine the best technique to extract antioxidants. The antioxidant capacity of the extracts was determined with DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. Pressurised fluid extraction (PFE) turned out to be the best technique and a response surface design was therefore utilised to optimise PFE. Furthermore, NMR and HPLC-DAD-MS/MS were applied to identify the extracted antioxidants. Results - PFE using water and ethanol as solvent at 160 and 180 degrees C, respectively, gave extracts of the highest antioxidant capacity. Stilbene glucosides such as isorhapontin, piceid and astringin were identified in the extracts. Conclusion - The study has shown that PFE is a fast and environmentally sustainable technique, using water and ethanol as solvent for the extraction of antioxidants from spruce bark. Copyright (C) 2011 John Wiley & Sons, Ltd.

Publishing year

2012

Language

English

Pages

1-11

Publication/Series

Phytochemical Analysis

Volume

23

Issue

1

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Organic Chemistry

Keywords

  • accelerated solvent extraction
  • antioxidant
  • DPPH
  • ethanol
  • Picea abies
  • pressurised fluid extraction
  • supercritical fluid extraction
  • water
  • stilbene glucosides

Status

Published

ISBN/ISSN/Other

  • ISSN: 1099-1565