Naturally occurring short splice variant of CYLD positively regulates dendritic cell function
Author
Summary, in English
Deubiquitination of NF-kappa B members by CYLD is crucial in controlling the magnitude and nature of cell activation. The role of the naturally occurring CYLD splice variant in dendritic cell (DC) function was analyzed using CYLDex7/8 mice, which lack the full-length CYLD (flCYLD) transcript and overexpress the short splice variant (sCYLD). Bone marrow-derived DCs from CYLDex7/8 mice display a hyperactive phenotype in vitro and in vivo and have a defect in establishing tolerance with the use of DEC-205-mediated antigen targeting to resting DCs. The combination of sCYLD overexpression and lack of flCYLD in CYLDex7/8 DCs leads to enhanced NF-kappa B activity accompanied by an increased nuclear translocation of the I kappa B molecule Bcl-3, along with nuclear p50 and p65. This suggests that, in contrast to flCYLD, sCYLD is a positive regulator of NF-kappa B activity, and its overexpression induces a hyperactive phenotype in DCs. (Blood. 2009; 113: 5891-5895)
Department/s
Publishing year
2009
Language
English
Pages
5891-5895
Publication/Series
Blood
Volume
113
Issue
23
Document type
Journal article
Publisher
American Society of Hematology
Topic
- Hematology
Status
Published
Research group
- Cell Pathology, Malmö
ISBN/ISSN/Other
- ISSN: 1528-0020