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Digital holographic microscopy for non-invasive monitoring of cell cycle arrest in l929 cells.

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Author

  • Maria Falck Miniotis
  • Anthonny Mukwaya
  • Anette Gjörloff Wingren

Summary, in English

Digital holographic microscopy (DHM) has emerged as a powerful non-invasive tool for cell analysis. It has the capacity to analyse multiple parameters simultaneously, such as cell- number, confluence and phase volume. This is done while cells are still adhered and growing in their culture flask. The aim of this study was to investigate whether DHM was able to monitor drug-induced cell cycle arrest in cultured cells and thus provide a non-disruptive alternative to flow cytometry. DHM parameters from G1 and G2/M cell cycle arrested L929 mouse fibroblast cells were collected. Cell cycle arrest was verified with flow cytometry. This study shows that DHM is able to monitor phase volume changes corresponding to either a G1 or G2/M cell cycle arrest. G1-phase arrest with staurosporine correlated with a decrease in the average cell phase volume and G2/M-phase arrest with colcemid and etoposide correlated with an increase in the average cell phase volume. Importantly, DHM analysis of average cell phase volume was of comparable accuracy to flow cytometric measurement of cell cycle phase distribution as recorded following dose-dependent treatment with etoposide. Average cell phase volume changes in response to treatment with cell cycle arresting compounds could therefore be used as a DHM marker for monitoring cell cycle arrest in cultured mammalian cells.

Publishing year

2014

Language

English

Publication/Series

PLoS ONE

Volume

9

Issue

9

Document type

Journal article

Publisher

Public Library of Science (PLoS)

Topic

  • Cancer and Oncology

Status

Published

Research group

  • Experimental Cancer Research, Malmö

ISBN/ISSN/Other

  • ISSN: 1932-6203