Retinoic Acid regulates hematopoietic development from human pluripotent stem cells.
Author
Summary, in English
The functions of retinoic acid (RA), a potent morphogen with crucial roles in embryogenesis including developmental hematopoiesis, have not been thoroughly investigated in the human setting. Using an in vitro model of human hematopoietic development, we evaluated the effects of RA signaling on the development of blood and on generated hematopoietic progenitors. Decreased RA signaling increases the generation of cells with a hematopoietic stem cell (HSC)-like phenotype, capable of differentiation into myeloid and lymphoid lineages, through two separate mechanisms: by increasing the commitment of pluripotent stem cells toward the hematopoietic lineage during the developmental process and by decreasing the differentiation of generated blood progenitors. Our results demonstrate that controlled low-level RA signaling is a requirement in human blood development, and we propose a new interpretation of RA as a regulatory factor, where appropriate control of RA signaling enables increased generation of hematopoietic progenitor cells from pluripotent stem cells in vitro.
Department/s
- Division of Molecular Medicine and Gene Therapy
- Hematopoietic Stem Cell Development
- Stem Cell Center
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
Publishing year
2015
Language
English
Pages
269-281
Publication/Series
Stem Cell Reports
Volume
4
Issue
2
Full text
Links
Document type
Journal article
Publisher
Cell Press
Topic
- Cell and Molecular Biology
Status
Published
Research group
- Hematopoietic Stem Cell Development
ISBN/ISSN/Other
- ISSN: 2213-6711