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Milk membrane lipid vesicle structures studied with Cryo-TEM.

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Author

Summary, in English

Milk fat globule membrane (MFGM) lipids have been studied in the presence and absence of proteins -lactoglobulin and -casein. The aim of this study was to relate the self-assembly structure, e.g. vesicles, formed in aqueous dispersions of MFGM lipids to the lipid composition, electrolyte composition as well as the effect of added milk proteins, i.e. -lactoglobulin and -casein. For this purpose, vesicles of phospholipid mixtures, containing dioleoylphosphatidylcholine (DOPC), sphingomyelin (SM), dioleoylphosphatidylethanolamine (DOPE), phosphatidylinositol (PI) and dioleoylphosphatidylserine (DOPS) at composition corresponding to that of the MFGM, were prepared by extrusion. The morphology of the formed structures of different sample compositions was studied with cryogenic transmission electron microscopy (Cryo-TEM). Mixtures of membrane lipid with a composition (e.g. 80% DOPE, 12% DOPC and 8% SM) that at high lipid content give liquid crystalline phases at the boundary of lamellar to reversed hexagonal phase rather formed microtubular structures than vesicles at high water content. A large proportion of multilamellar vesicles is formed in buffer and divalent salts than in pure water. A small increase in the interlayer spacing of the multilamellar vesicle was observed in the presence of -casein.

Department/s

Publishing year

2003

Language

English

Pages

257-264

Publication/Series

Colloids and Surfaces B: Biointerfaces

Volume

31

Issue

1-4

Document type

Journal article

Publisher

Elsevier

Topic

  • Physical Chemistry

Keywords

  • Vesicles
  • Protein
  • Membrane lipids
  • Vesicle structures
  • Cryo-TEM

Status

Published

ISBN/ISSN/Other

  • ISSN: 1873-4367