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Microfluidic impedance cytometer for platelet analysis.

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Author

  • Mikael Evander
  • Antonio J Ricco
  • John Morser
  • Gregory T A Kovacs
  • Lawrence L K Leung
  • Laurent Giovangrandi

Summary, in English

We present the design and performance characteristics of a platelet analysis platform based on a microfluidic impedance cytometer. Dielectrophoretic focusing is used to centre cells in a fluid stream, which then forms the core of a two-phase flow (dielectric focusing). This flow then passes between electrodes for analysis by differential impedance spectroscopy at multiple frequencies from 280 kHz to 4 MHz. This approach increases the signal-to-noise ratio relative to a single-phase, unfocused stream, while minimising the shear forces to which the cells are subjected. The percentage of activated platelets before and after passage through the chip was measured using flow cytometry, and no significant change was measured. Measuring the in-phase amplitude at a single frequency is sufficient to distinguish platelets from erythrocytes. Using multi-frequency impedance measurements and discriminant analysis, resting platelets can be discriminated from activated platelets. This multifrequency impedance cytometer therefore allows ready determination of the degree of platelet activation in blood samples.

Publishing year

2013

Language

English

Pages

722-729

Publication/Series

Lab on a Chip

Volume

13

Issue

4

Document type

Journal article

Publisher

Royal Society of Chemistry

Topic

  • Medical Engineering

Status

Published

ISBN/ISSN/Other

  • ISSN: 1473-0189