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Sorting of neutrophil-specific granule protein human cathelicidin, hCAP-18, when constitutively expressed in myeloid cells.

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Author

Summary, in English

Neutrophil granulocytes carry storage organelles, e.g., azurophil and specific granules. Poorly understood are the mechanisms for retrieval from constitutive secretion followed by sorting for storage. Therefore, we asked whether the specific granule protein human cathelicidin (hCAP-18) could be sorted for storage in other granules when the biosynthetic window is widened to allow this. We observed that hCAP-18 was targeted for storage in lysosome-related organelles when expressed constitutively in the rat basophilic leukemia and the mouse promyelocytic (MPRO) cell lines. In addition, premature release of the antibiotic C-terminal peptide LL-37 was observed. Retention of hCAP-18 was diminished by induction of differentiation of MPRO cells. In conclusion, a specific granule protein with native conformation may be sorted for storage in lysosome-related organelles of myeloid cells and converted prematurely to a supposedly biologically active form.

Publishing year

2002

Language

English

Pages

147-153

Publication/Series

Journal of Leukocyte Biology

Volume

72

Issue

1

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Cell and Molecular Biology

Keywords

  • Cytoplasmic Granules : metabolism
  • Cytoplasmic Granules : ultrastructure
  • Mast Cells : metabolism
  • Lysosomes : metabolism
  • Mast Cells : ultrastructure
  • Myeloid Cells : ultrastructure
  • Myeloid Cells : metabolism
  • Mice
  • Myeloid Progenitor Cells : metabolism
  • Neutrophils : chemistry
  • Protein Transport
  • Rats
  • Support
  • Non-U.S. Gov't
  • Transfection
  • Cell Line
  • Antimicrobial Cationic Peptides : metabolism
  • Antimicrobial Cationic Peptides : genetics
  • Antimicrobial Cationic Peptides : analysis
  • Animal

Status

Published

ISBN/ISSN/Other

  • ISSN: 1938-3673