Generating regionalized neuronal cells from pluripotency, a step-by-step protocol.
Author
Summary, in English
Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.
Department/s
Publishing year
2012
Language
English
Publication/Series
Frontiers in Cellular Neuroscience
Volume
6
Issue
Published online: 03 January 2013
Full text
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Links
Document type
Journal article
Publisher
Frontiers Media S. A.
Topic
- Neurosciences
Status
Published
Research group
- Developmental and Regenerative Neurobiology
ISBN/ISSN/Other
- ISSN: 1662-5102