Porous protein-based scaffolds prepared through freezing as potential scaffolds for tissue engineering.
Author
Summary, in English
Successful tissue engineering with the aid of a polymer scaffold offers the possibility to produce a larger construct and to mould the shape after the defect. We investigated the use of cryogelation to form protein-based scaffolds through different types of formation mechanisms; enzymatic crosslinking, chemical crosslinking, and non-covalent interactions. Casein was found to best suited for enzymatic crosslinking, gelatin for chemical crosslinking, and ovalbumin for non-covalent interactions. Fibroblasts and myoblasts were used to evaluate the cryogels for tissue engineering purposes. The stability of the cryogels over time in culture differed depending on formation mechanism. Casein cryogels showed best potential to be used in skeletal tissue engineering, whereas gelatin cryogels would be more suitable for compliable soft tissues even though it also seemed to support a myogenic phenotype. Ovalbumin cryogels would be better suited for elastic tissues with faster regeneration properties due to its faster degradation time. Overall, the cryogelation technique offers a fast, cheap and reproducible way of creating porous scaffolds from proteins without the use of toxic compounds.
Department/s
Publishing year
2012
Language
English
Pages
2489-2498
Publication/Series
Journal of Materials Science: Materials in Medicine
Volume
23
Issue
10
Document type
Journal article
Publisher
Springer
Topic
- Medical Materials
Status
Published
Research group
- Muscle Biology
ISBN/ISSN/Other
- ISSN: 1573-4838