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Direct assessment of microbial colonisation in damp houses by chemical marker analysis

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Author

Summary, in English

Ergosterol was used as a marker of fungal biomass and 3-hydroxy fatty acids were used as markers of bacterial endotoxin to determine microbial colonisation of building materials by gas chromatography-mass spectrometry. Pieces (0.3-0.5 g) of materials from two water-damaged buildings were hydrolysed to liberate the markers from larger microbial structures; the preparations were then purified, derivatised, and analysed. The marker patterns obtained revealed that the microbial communities differed between the two buildings both qualitatively and quantitatively. The chemical-analytical method described should be useful for detecting and characterising microbial colonisation on surfaces in, for example, damp buildings.

Publishing year

2003

Language

English

Pages

251-254

Publication/Series

Indoor & Built Environment

Volume

12

Issue

4

Document type

Journal article

Publisher

SAGE Publications

Topic

  • Microbiology in the medical area

Keywords

  • fungi
  • bacteria
  • ergosterol
  • 3-hydroxy fatty acids
  • building materials

Status

Published

ISBN/ISSN/Other

  • ISSN: 1420-326X