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Adipocytes derived from PA6 cells reliably promote the differentiation of dopaminergic neurons from human embryonic stem cells.

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Author

  • Oktar Guloglu
  • Anna Larsen
  • Patrik Brundin

Summary, in English

The PA6 stromal cell line comprises a heterogeneous population of cells that can induce both mouse and human embryonic stem cells to differentiate into dopaminergic neurons. This ability of PA6 cells has been termed stromal cell-derived inducing activity (SDIA). The level of SDIA has been found to vary considerably between and within batches of PA6 cells. Not only are the molecular mechanisms that underlie SDIA unknown but also the cell type(s) within the heterogeneous PA6 cultures that underlie SDIA remain poorly defined. In this study, we reveal that adipocytes, which are present within the heterogeneous PA6 cell population, robustly release the factors mediating SDIA. Furthermore, we report that the coculture of human embryonic stem cells with PA6-derived adipocytes reliably induces their differentiation into midbrain dopaminergic neurons. © 2014 Wiley Periodicals, Inc.

Publishing year

2014

Language

English

Pages

564-573

Publication/Series

Journal of Neuroscience Research

Volume

92

Issue

5

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Neurosciences

Status

Published

ISBN/ISSN/Other

  • ISSN: 1097-4547