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HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease.

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Author

  • Ewa H Lavant
  • Joyce Carlson

Summary, in English

The risk for celiac disease (CD) is clearly related to specific HLA DQA1 and DQB1 alleles, but HLA -typing is often considered too costly for frequent use.Here we present a method using sequence-specific primed PCR (PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresis on Applied Biosystems 3130xl Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step, this method reduces the reagent expenses and technical time for directed HLA typing to distinguish risk alleles for CD, with a sufficient throughput for large-scale screening projects.

Publishing year

2013

Language

English

Pages

297-307

Publication/Series

Methods in Molecular Biology

Volume

919

Document type

Journal article

Publisher

Springer

Topic

  • Pharmacology and Toxicology
  • Medicinal Chemistry

Status

Published

ISBN/ISSN/Other

  • ISSN: 1940-6029