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Non-pylori Helicobacteraceae in the upper digestive tract of asymptomatic venezuelan subjects: Detection of helicobacter cetorum-like and candidatus Wolinella africanus-like DNA

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Author

  • M. Alexandra Garcia-Amado
  • Waleed Abu Al-Soud
  • Pedro Borges-Landaez
  • Monica Contreras
  • Samandhy Cedeno
  • Estalina Baez-Ramirez
  • M. Gloria Dominguez-Bello
  • Torkel Wadström
  • Pulcherie Gueneau

Summary, in English

Background: The spectrum of human non-pylori Helicobacter infections is expanding, with species such as H. heilmannii and H. felis occasionally being associated with gastritis. However, the existence of non-pylori Helicobacter colonization in asymptomatic subjects has not been evaluated. The aim of this study was to investigate whether Helicobacter species other than pylori are present in the upper digestive tract of asymptomatic human subjects. Materials and methods: A Helicobacteraceae-specific semi-nested polymerase chain reaction (PCR) assay was used to detect Helicobacter-like organisms in the upper digestive tract of 91 Venezuelan volunteers (aged 18-68 years, 41 females, 50 males). Species were identified by denaturing gradient gel electrophoresis analysis and sequencing of the PCR products. Results: We detected DNA sharing 99-100% sequence identity in over 300-400 bp with the 16S rRNA genes of H. pylori, H. cetorum, and Candidatus Wolinella africanus in 76%, 16%, and 15% of the subjects, respectively. Multiple colonization was documented in 10% of the subjects: H. cetorum and Candidatus W. africanus (4%), H. pylori and Candidatus W. africanus (4%), and H. pylori and H. cetorum (2%). Conclusions: Our results suggest that non-pylori Helicobacteraceae colonization is relatively common in the Venezuelan asymptomatic population. This is the first report documenting the presence of H. cetorum DNA in the human upper digestive tract, and the second report of the recently discovered Candidatus W. africanus.

Publishing year

2007

Language

English

Pages

553-558

Publication/Series

Helicobacter

Volume

12

Issue

5

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Microbiology in the medical area

Keywords

  • PCR
  • Helicobacteraceae
  • prevalence

Status

Published

ISBN/ISSN/Other

  • ISSN: 1083-4389