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Detection of neurocan in cerebrospinal fluid.

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Author

Summary, in English

Cerebrospinal fluid (CFS) is the most easily accessible component of the human central nervous system and has been successfully used for the analysis of disease-associated molecular imbalances, particularly for extracellular matrix components. Alterations in the presence of the nervous system-associated chondroitin sulfate proteoglycan neurocan had been reported from active multiple sclerosis lesions. Neurocan could be detected as a component of human CFS after enrichment of proteoglycans by anion exchange chromatography from pooled liquor as well as individual 300 μL samples by Western blot. However, a general alteration in neurocan levels in CFS sample with high immunoglobulin content could not be demonstrated. To further reduce the sample size, the development of a PG capturing assay based on polybrene-coated 96-well plates was initiated. This approach could be an interesting alternative option for the analysis of PGs in biological fluid and tissue samples.

Publishing year

2012

Language

English

Pages

87-95

Publication/Series

Methods in Molecular Biology

Volume

836

Document type

Journal article

Publisher

Springer

Topic

  • Cell and Molecular Biology

Status

Published

Research group

  • Vessel Wall Biology

ISBN/ISSN/Other

  • ISSN: 1940-6029