SPI-C, a PU-box binding ETS protein expressed temporarily during B-cell development and in macrophages, contains an acidic transactivation domain located to the N-terminus.

Summary, in English

Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription factor closely related to PU.1 and SPI-B, and expressed temporarily during B-cell development and in macrophages. By deletion and mutation analysis we show that the SPI-C protein has a transactivation domain located to the N-terminus, and that the transactivation activity is reduced to that of the DNA binding domain (DBD) alone when four aspartic acid residues are mutated to alanines. PU.1 and SPI-B regulate transcription from acidic domains located to the N-terminus and by recruiting the co-activator PIP to adjacent sites in a sequence specific manner. In contrast to PU.1 and PIP, SPI-C and PIP were unable to form a distinct ternary complex on the Ig λ light chain λ2–4 enhancer element, suggesting that SPI-C could act both as a positive and negative transcriptional regulator during B-lymphocyte differentiation.

• Author: Robert Carlsson; Christine Persson; Tomas Leanderson
• Department/s: Immunology; Breastcancer-genetics
• Publishing year: 2003
• Language: English
• Pages: 1035-1043
• Publication/Series: Molecular Immunology
• Volume: 39
• Issue: 16
• Links: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12749910&dopt=Abstract; http://dx.doi.org/10.1016/S0161-5890(03)00032-4
• Document type: Journal article
• Publisher: Pergamon Press Ltd.
• Topic: Immunology in the medical area
• Keywords: Transcription; PU.1; His-tag; Modular; Substitution
• Status: Published
• Research group: Immunology
• ISBN/ISSN/Other: ISSN: 1872-9142