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Cell cycle phase-dependent induction of ornithine decarboxylase-antizyme.

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Author

  • Margareta Linden
  • Siw Anehus
  • Eva Långström-Einarsson
  • Bo Baldetorp
  • Olle Heby

Summary, in English

The activites of ornithine decarboxylase (ODC) and ODC inhibitory protein (ODC-antizyme) were studied in Ehrlich ascites tumor cells, separated according to their position in the cell cycle by centrifugal elutriation. Release and/or synthesis of ODC-antizyme was induced by putrescine treatment. Each mouse received an intraperitoneal injection of 25 moles of putrescine at 0, 1, 2, and 3 hr after tumor transplantation. Tumor cells obtained from putrescine-treated and control mice at 4 hr after transplantation were separated into fractions representing all phases of the cell cycle. The cell cycle distribution of the tumor cells in each fraction was determined by flow cytometry.

In control tumor cells the ODC activity exhibited two maxima; inlate-G1/early-S and in late-S/G2. A marked decrease in ODC activity was observed in mid-S phase. This decrease coincided with maximum ODC-antizyme activity (revealed by putrescine treatment), suggesting that ODC-antizyme is involved in the regulation of ODC activity during the cell cycle.

Publishing year

1985

Language

English

Pages

273-276

Publication/Series

Journal of Cellular Physiology

Volume

125

Issue

2

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Cancer and Oncology

Status

Published

ISBN/ISSN/Other

  • ISSN: 1097-4652