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Evaluation of a Probing Feeding Strategy in Large Scale Cultivations

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Author

  • Stéphane Velut
  • Lena de Maré
  • Jan Peter Axelsson
  • Per Hagander

Summary, in English

This report presents the results of two \emph{Escherichia~coli} cultivations performed in a large scale industrial bioreactor. The experiments aimed at evaluating a probing feeding strategy that adresses the problem of acetate accumulation. The principle is to superimpose short pulses in the glucose feed and evaluate the response in the dissolved oxygen signal for the feed adjustments. Simulations of a two compartments system are also done to give insight into the possible effects of the scale and the imperfect mixing on the feeding strategy. Satisfactory results in terms of recombinant protein amounts have been obtained and no major adverse large scale effects were observed.

Publishing year

2002

Language

English

Publication/Series

Technical Reports TFRT-7601

Document type

Report

Publisher

Department of Automatic Control, Lund Institute of Technology (LTH)

Topic

  • Control Engineering

Status

Published

ISBN/ISSN/Other

  • ISSN: 0280-5316