Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family

Summary, in English

We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family, The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. (C) 2000 Academic Press.

• Author: U Aapola; K Shibuya; HS Scott; J Ollila; Mauno Vihinen; M Heino; A Shintani; K Kawasaki; S Minoshima; K Krohn; SE Antonarakis; N Shimizu; J Kudoh; P Peterson
• Publishing year: 2000
• Language: English
• Pages: 293-298
• Publication/Series: Genomics
• Volume: 65
• Issue: 3
• Links: http://dx.doi.org/10.1006/geno.2000.6168
• Document type: Journal article
• Publisher: Academic Press
• Topic: Medical Genetics
• Status: Published
• ISBN/ISSN/Other: ISSN: 1089-8646