Selective Generation of Gut Tropic T Cells in Gut-associated Lymphoid Tissue (GALT): Requirement for GALT Dendritic Cells and Adjuvant.
Author
Summary, in English
n the current study, we address the underlying mechanism for the selective generation of gut-homing T cells in the gut-associated lymphoid tissues (GALT). We demonstrate that DCs in the GALT are unique in their capacity to establish T cell gut tropism but in vivo only confer this property to T cells in the presence of DC maturational stimuli, including toll-like receptor-dependent and -independent adjuvants. Thus, DCs from mesenteric LNs (MLNs), but not from spleen, supported expression of the chemokine receptor CCR9 and integrin {alpha}4ß7 by activated CD8+ T cells. While DCs were also required for an efficient down-regulation of CD62L, this function was not restricted to MLN DCs. In an adoptive CD8+ T cell transfer model, antigen-specific T cells entering the small intestinal epithelium were homogeneously CCR9+{alpha}4ß7+CD62Llow, and this phenotype was only generated in GALT and in the presence of adjuvant. Consistent with the CCR9+ phenotype of the gut-homing T cells, CCR9 was found to play a critical role in the localization of T cells to the small intestinal epithelium. Together, these results demonstrate that GALT DCs and T cell expression of CCR9 play critical and integrated roles during T cell homing to the gut.
Department/s
- Immunology
Publishing year
2003
Language
English
Pages
963-969
Publication/Series
Journal of Experimental Medicine
Volume
198
Issue
6
Full text
Links
Document type
Journal article
Publisher
Rockefeller University Press
Topic
- Immunology in the medical area
Keywords
- lymphocytes
- antigen-presenting cell
- inflammation
- chemokines
- intestinal mucosa
Status
Published
Research group
- Immunology
ISBN/ISSN/Other
- ISSN: 1540-9538