Method for ultrastructural studies of the intact tissue-metal interface
Author
Summary, in English
Samples were prepared for ultrastructural studies of the intact interface between metallic implants and tissue by transmission electron microscopy. The method is based on plastic embedding of implant and tissue and subsequent removal of the bulk metal by electrochemical dissolution (electropolishing), to facilitate preparation of ultrathin sections for transmission electron microscopy. Surface sensitive spectroscopy (Auger electron microscopy and X-ray photoemission spectroscopy) and transmission electron microscopy EDX results show that the method produces samples with an intact interface, containing the implant surface oxide and the adjacent tissue. Examples of application of the method on titanium, zirconium and aluminium implants in soft tissue are given.
Publishing year
1990
Language
English
Pages
596-601
Publication/Series
Biomaterials
Volume
11
Issue
8
Document type
Journal article
Publisher
Elsevier
Topic
- Bioengineering Equipment
Keywords
- Titanium
- cell-material interface
- ultrastructure
Status
Published
ISBN/ISSN/Other
- ISSN: 1878-5905