Acoustophoretic microfluidic chip for sequential elution of surface bound molecules from beads or cells
Author
Summary, in English
An acoustophoresis-based microfluidic flow-chip is presented as a novel platform to facilitate analysis of proteins and peptides loosely bound to the surface of beads or cells. The chip allows for direct removal of the background surrounding the beads or cells, followed by sequential treatment and collection of a sequence of up to five different buffer conditions. During this treatment, the beads/cells are retained in a single flow by acoustic radiation force. Eluted peptides are collected from the outlets and subsequently purified by miniaturized solid-phase extraction and analyzed with matrix assisted laser desorption mass spectrometry. Fundamental parameters such as the system fluidics and dispersion are presented. The device was successfully applied for wash and sequential elution of peptides bound to the surface of microbeads and human spermatozoa, respectively. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4749289]
Department/s
- Department of Biomedical Engineering
- Clinical Chemistry, Malmö
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
Publishing year
2012
Language
English
Publication/Series
Biomicrofluidics
Volume
6
Issue
3
Full text
Document type
Journal article
Publisher
American Institute of Physics (AIP)
Topic
- Medicinal Chemistry
Status
Published
Research group
- Clinical Chemistry, Malmö
ISBN/ISSN/Other
- ISSN: 1932-1058